Autoclave was invented by Charles Chamberland, in 1879, and perfected by Yorco in the middle of the 20th century in 1965 and absolutely latest features are continuously being edit. Autoclave is used to sterilize all surgical equipment, Pharmaceutical accessories, Lab instrument needing Sterilization power of steam is used to kill bacteria, spores, and microbes Resistance to boiling water. Steam is heated to 121°C which is above the boiling point of water which is 100°C and very effectively sterilized the item.